Diagnosing Vaginal Infections
A drop of sterile saline is placed over a clean glass slide,
A sample of the vaginal discharge is acquired over a sterile swab,
The specimen material in the swab is gently brought in contact with the sterile saline and stirred to let them mix,
Almost immediately the preparation is covered with the glass coverslip so that it DOES NOT dry up,
The above wet mount is investigated under a CLM in a rather reduced illumination (Do not use oil immersion lens for wet mounts),
Observation: The CM magnifications may normally reveal the following possible causes of infection:
Presence of yeast cells, indicating colonies of yeast vaganities,
If motile trophozoites of T. vaginalies are seen, the presence of trichomoniasis may be presumed,
If an accumulation of clue cells (squamous epithelial cells occupied with colonies of cocobacilli) is witnessed, will indicate a bacterial vaginosis.
In the above case a Gram stain preparation of the vaginal fluid is recommended and its inspection under the CM may specify the following results:
Presence of some Gram-positive bacilli or Lactobacillus spp.
Presence of many Gram-negative bacilli or Bacteroids spp. Many curved Gram-negative bacilli or Mobiluncus spp.
Presence of Gram-variable coccobacilli or Gardnerella vaginalis.