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Comparison of Eight Different Types of Microscopes (Page 2 of 2)
Article by
Robyn Broyles
(11,124 pts
)
, published Oct 4, 2009
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2 comments
5) USB microscope
Magnification:
Up to 200x.
Depth of field:
Relatively small.
Uses:
Document examination, detail work, hobbies, education, entertainment
Advantages:
Can be used on almost any object and requires no preparation of the specimen. Inexpensive. Can usually be purchased for under $130 USD.
Disadvantages:
Not well suited to the same scientific applications as other optical microscopes.
6) Transmission electron microscope
Magnification:
A series of electrostatic and electromagnetic lenses act on an electron beam to produce up to 50 million times magnification
Depth of field:
Very small.
Uses:
Microbiology, pathology, crystallography
Advantages:
Very high resolution.
Disadvantages:
Requires extensive specimen preparation, including staining and sectioning with an ultramicrotome. Cannot observe the surface of objects.Very specialized equipment that requires a partial vacuum.
7) Scanning electron microscope
Magnification:
Electrostatic and electromagnetic lenses, as with a TEM. Magnification ranges from 25x to 250,000x.
Depth of field:
Large compared to that of a transmission electron microscope.
Uses:
Biology, microbiology, geology, nanotechnology, crystallography
Advantages:
Can view objects' three-dimensional surface. Also, an SEM can provide information about the specimen's elemental composition.
Disadvantages:
As with TEM, requires specialized equipment and a partial vacuum. Cannot be used on living specimens.
8) Scanning probe microscope
Magnification:
Uses no lenses at all. Instead, the image is formed by making physical contact with a specimen with a probe, which moves across the specimen in a raster pattern. Scanning probe microscopes can detect features as small as a few picometers in width. (A picometer is a trillionth of a meter, or a thousandth of a nanometer.)
Depth of field:
Varies according to technique.
Uses:
Nanotechnology, biology, microbiology, molecular biology, pathology, food research
Advantages:
Unparalleled resolution. The physical interaction between the probe and specimen can be used to change the specimen, for example by etching it, in a process called nanolithography.
Disadvantages:
Image acquisition is slow and the image size is small. Solid-solid and liquid-liquid interfaces provide a challenge.
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Comments
Jul 8, 2009 1:26 AM
M B Awan
OPTICAL & STEREO MICROSCOPES
Please let me about the subject items. If I forward the requred specifications of the same can you help me to opt the correct and the best one?
OPTICAL MICROSCOPE:
Basic Magnification: 100x - 1000x
Eye pieces : WF 10xeyepieces paired (field of view
Thanks
Jun 24, 2009 12:03 AM
bilat
lol.....
hey you your eight kinds of microscope is wrong.....you don't have the compound and comparison microscope....
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