http://wordpress.org/extend/plugins/nospamnx/

click to enlarge
If you've ever commented on a blog using a captcha spam-blocking system, you know how annoying that is. Instead of inconveniencing your readers, why not stop comment spam in a more...let's say, STEALTHY manner? One WordPress commenter blacklist plugin that does just this is NoSpamNX.
NoSpamNX uses CSS to add invisible fields to your comment form. If these fields somehow get filled out, that raises a red flag for NoSpamNX and the comments as well as the commenters get blacklisted. Now who would fill out invisible fields anyway? That's right, spambots will. And spambots will get blocked.
Image Credit: Screenshot ©NoSpamNX